UK firms have accused the government of leaving them "hung out to dry" in the event of a no-deal Brexit.
With less than 50 days until 29 March when the UK is due to leave the EU, the British Chambers of Commerce (BCC) says 20 key questions remain unresolved.
How to move skilled staff between the UK and EU, which rules to follow, and what trade deals will be in place are all still unknown, the BCC says.
The government said it was focused on getting approval for its Brexit deal.
"I absolutely recognise that for many businesses it is a period of uncertainty and concern," Brexit Secretary Stephen Barclay told BBC Radio 4's Today Programme. "That's why we are so committed to securing a deal."
He said he had already met BCC representatives to discuss the list of key questions.
On the matter of trade tariffs in the event of a no-deal Brexit, he said he expected to be able to give more information "in the coming days".
Theresa May is currently seeking changes to her Brexit deal with the EU after it was emphatically rejected last month, in the largest defeat ever for a sitting government.
The prime minister needs to get a deal approved by Parliament by 29 March to avoid a no-deal Brexit. In that case, in the countries where the UK had no formal trade agreement, both would have to trade under the rules overseen by the World Trade Organization (WTO).
Under this system, every WTO member is free to negotiate its own tariffs - or taxes - on different goods. But under the rules, members have to offer the same tariff to every other WTO country.
The UK has signed "continuity agreements", which mean there will be no disruption to trade, with Switzerland, Chile, The Faroe Islands and Eastern and Southern Africa. As a result free trade agreements currently in place between the EU and those countries will apply to the UK after Brexit.
Mutual recognition agreements - where a product lawfully sold in one country can be sold in another - have also been signed with Australia and New Zealand.